Spectrophotometrically, Spectroscopically, Microscopically and Thermogravimetrically Optimized TiO2 and ZnO Nanoparticles and their Bactericidal, Antioxidant and Cytotoxic Potential: A Novel Comparative Approach

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Abstract
Current study was aimed to determine the antibacterial, antioxidant and cytotoxic potential of Titanium dioxide nanoparticles (TiO2NPs) and Zinc oxide nanoparticles (ZnONPs). Nanoparticles were characterized by UV–Vis spectrophotometry, particle size analyzer (PSA), fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The Minimum inhibitory concentration (MIC) was determined by standard agar dilution method. Antibacterial potential of nanoparticles was analyzed by standard disc diffusion method against bacterial strains including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia. Different concentrations of NPs (0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4 mg/mL) were incorporated to evaluate the antimicrobial activity. Antioxidant activity and cytotoxicity of these NPs was analyzed by DPPH method and brine shrimp cytotoxicity assay, respectively. The MIC of TiO2NPs against E. coli, P. aeruginosa and K. pneumoniae was 0.04, 0.08 and 0.07 mg/mL respectively while the MIC of ZnONPs against the above strains was 0.01, 0.015 and 0.01 mg/mL. The maximum zone of inhibition was observed for K. pneumoniae i.e., 20mm and 25mm against TiO2 and ZnO NPs respectively, at 1.4 mg/mL concentration of NPs. The susceptibility of NPs against bacterial strains was evaluated in the following order: K. pneumoniae > P. aeruginosa > E. coli. The antioxidant activity of nanoparticles increased by increasing the concentration of NPs while cytotoxic analysis exhibited non-toxic effect of ZnO NPs while TiO2 had toxic effects on 1.2 and 1.4 mg/mL concentrations. Results revealed that ZnO NPs have more antibacterial and negligible cytotoxic potential in contrast to TiO2 NPs.

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