Live imaging of nervous system development and function using light‐sheet microscopy

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Abstract
SUMMARY: In vivo imaging applications typically require carefully balancing conflicting parameters. Often it is necessary to achieve high imaging speed, low photo‐bleaching, and photo‐toxicity, good three‐dimensional resolution, high signal‐to‐noise ratio, and excellent physical coverage at the same time. Light‐sheet microscopy provides good performance in all of these categories, and is thus emerging as a particularly powerful live imaging method for the life sciences. We see an outstanding potential for applying light‐sheet microscopy to the study of development and function of the early nervous system in vertebrates and higher invertebrates. Here, we review state‐of‐the‐art approaches to live imaging of early development, and show how the unique capabilities of light‐sheet microscopy can further advance our understanding of the development and function of the nervous system. We discuss key considerations in the design of light‐sheet microscopy experiments, including sample preparation and fluorescent marker strategies, and provide an outlook for future directions in the field. Mol. Reprod. Dev. 82: 605–618, 2015. © 2013 Wiley Periodicals, Inc.
Funding Information
  • Howard Hughes Medical Institute

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