Co‐expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab′ fragment expressed inEscherichia coli
Authors:
- Neil WeirNWNeil Weir
- Alastair LawsonALAlastair Lawson
- Andrew MountainAMAndrew Mountain
- Peter A. LundPLPeter A. Lund
- 12 February 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 380 (1-2) , 194-197
Abstract
Secretion to the periplasm of Escherichia coli enables production of many eukaryotic extracellular proteins in a soluble form. The complex disulphide bond arrangement of such proteins is probably a major factor in determining the low yield of correctly folded product observed in many cases. Here we show that co-expression of human protein disulphide isomerase increased the yield of a monoclonal antibody Gab′ fragment in the periplasm of E. coliKeywords
This publication has 15 references indexed in Scilit:
- Building bridges: disulphide bond formation in the cellMolecular Microbiology, 1994
- CaBP1, a calcium binding protein of the thioredoxin family, is a resident KDEL protein of the ER and not of the intermediate compartmentJournal of Cell Science, 1994
- A model catalyst of protein disulphide bond formationCurrent Biology, 1993
- The Reported cDNA Sequence for Phospholipase Cα Encodes Protein Disulfide Isomerase, Isozyme Q-2 and Not Phospholipase CBiochemical and Biophysical Research Communications, 1993
- The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coliNature Biotechnology, 1993
- Identification of a protein required for disulfide bond formation in vivoCell, 1991
- ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase.Journal of Biological Chemistry, 1990
- Growth at sub-optimal temperatures allows the production of functional, antigen-binding Fab fragments in Escherichia coliGene, 1989
- Protein disulfide isomerase: Multiple roles in the modification of nascent secretory proteinsCell, 1989
- In vivo cross-linking of protein disulfide isomerase to immunoglobulinsBiochemistry, 1987