Cysteamine–palladium complex ([Pd(?-OAc)(ppy)] 2 , ppy:2-phenylpyridine, PhMe)-modified peroxidase biosensor immobilized on a gold electrode
Authors:
- 25 July 2013
- journal article
- Published by Taylor & Francis in Artificial Cells, Nanomedicine, and Biotechnology
- Vol. 42 (6) , 413-417
Abstract
A new peroxidase biosensor was developed using cysteamine–palladium complex-modified gold electrode. The principle of the measurements is based on monitoring increase in the oxidation potential of palladium complex (at + 0.47 V vs Ag/AgCl) using amperometric detection. In the optimization studies of the biosensor, effects of enzyme amount, palladium complex amount, and duration of SAM formation on biosensor responses were investigated to optimize the bioactive layer. The biosensor has a fast response time of less than 10 s to hydrogen peroxide (H2O2), with a linear range of 5.0 × 10− 6 to 150 × 10− 6 M and a detection limit of 3.38 × 10− 6 M.Keywords
This publication has 27 references indexed in Scilit:
- Iron-enriched natural zeolite modified carbon paste electrode for H2O2 detectionElectrochimica Acta, 2010
- Synthesis, X-ray structures, and catalytic activities of (κ2-C,N)-palladacycles bearing imidazol-2-ylidenesJournal of Organometallic Chemistry, 2009
- Effect of cysteine mutations on direct electron transfer of horseradish peroxidase on goldBiosensors and Bioelectronics, 2002
- Hydrogen Peroxide Biosensors Based on Direct Electron Transfer from Plant Peroxidases Immobilized on Self-Assembled Thiol-Monolayer Modified Gold ElectrodesElectroanalysis, 2001
- Chemiluminescent flow sensor for H2O2 based on the decomposition of H2O2 catalyzed by cobalt(II)-ethanolamine complex immobilized on resinAnalytica Chimica Acta, 2001
- Synthesis and characterization of a homologous series of mononuclear palladium complexes containing different cyclometalated ligandsInorganica Chimica Acta, 2000
- Direct electron transfer between heme-containing enzymes and electrodes as basis for third generation biosensorsAnalytica Chimica Acta, 1999
- An Enzyme Switch Employing Direct Electrochemical Communication between Horseradish Peroxidase and a Poly(aniline) FilmAnalytical Chemistry, 1998
- Amperometric sensors for peroxide, choline, and acetylcholine based on electron transfer between horseradish peroxidase and a redox polymerAnalytical Chemistry, 1993
- Electrical wiring of redox enzymesAccounts of Chemical Research, 1990