Simultaneous multiplexed amplicon sequencing and transcriptome profiling in single cells
- 17 December 2018
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 16 (1) , 59-62
Abstract
We describe droplet-assisted RNA targeting by single-cell sequencing (DART-seq), a versatile technology that enables multiplexed amplicon sequencing and transcriptome profiling in single cells. We applied DART-seq to simultaneously characterize the non-A-tailed transcripts of a segmented dsRNA virus and the transcriptome of the infected cell. In addition, we used DART-seq to simultaneously determine the natively paired, variable region heavy and light chain amplicons and the transcriptome of B lymphocytes.Keywords
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This publication has 30 references indexed in Scilit:
- Genetic measurement of memory B-cell recall using antibody repertoire sequencingProceedings of the National Academy of Sciences, 2013
- Correlation of Gene Expression and Genome Mutation in Single B-CellsPLOS ONE, 2013
- High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoireNature Biotechnology, 2013
- Reovirus Replication Protein μ2 Influences Cell Tropism by Promoting Particle Assembly within Viral InclusionsJournal of Virology, 2012
- Advances in Human B Cell Phenotypic ProfilingFrontiers in Immunology, 2012
- The Sequence Alignment/Map format and SAMtoolsBioinformatics, 2009
- A Plasmid-Based Reverse Genetics System for Animal Double-Stranded RNA VirusesCell Host & Microbe, 2007
- Reovirus Core Protein μ2 Determines the Filamentous Morphology of Viral Inclusion Bodies by Interacting with and Stabilizing MicrotubulesJournal of Virology, 2002
- Genome Replication and Packaging of Segmented Double-Stranded RNA VirusesVirology, 2000
- ImmunsubstitutionVerhandlungen der Deutschen Gesellschaft für innere Medizin, 1978