Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Top Cited Papers
Open Access
- 2 March 2014
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 11 (4) , 410-412
Abstract
The combination of stimulated Raman scattering (SRS) microscopy with an alkyne group as a Raman-active tag allows high-contrast, live-cell dynamic imaging of a variety of biomolecules including DNA, RNA, protein, lipids and small-molecule drugs. Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.Keywords
This publication has 19 references indexed in Scilit:
- Bioorthogonal Chemical Reporters for Analyzing Protein Lipidation and Lipid TraffickingAccounts of Chemical Research, 2011
- Coherent Nonlinear Optical Imaging: Beyond Fluorescence MicroscopyAnnual Review of Physical Chemistry, 2011
- Macrophages in the Pathogenesis of AtherosclerosisCell, 2011
- Imaging of EdU, an Alkyne-Tagged Cell Proliferation Probe, by Raman MicroscopyJournal of the American Chemical Society, 2011
- Metabolic labeling and direct imaging of choline phospholipids in vivoProceedings of the National Academy of Sciences, 2009
- Handbook of Biomedical Nonlinear Optical MicroscopyJournal of Biomedical Optics, 2009
- Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering MicroscopyScience, 2008
- Exploring RNA transcription and turnover in vivo by using click chemistryProceedings of the National Academy of Sciences, 2008
- A chemical method for fast and sensitive detection of DNA synthesis in vivoProceedings of the National Academy of Sciences, 2008
- Copper-free click chemistry for dynamic in vivo imagingProceedings of the National Academy of Sciences, 2007