Modifications in the Phenolphthalein Method for Spectrophotometric Estimation of Beta Cyclodextrin
Authors:
- 1 January 1995
- journal article
- research article
- Published by Wiley in Starch - Stärke
- Vol. 47 (10) , 399-400
Abstract
The phenolphthalein solution used in the assay of β‐Cyclodextrin is not stable and undergoes spontaneous decolorization. There is linear decrease in absorbance of phenolphthalein with time. This causes a continuous increase in error with time which is as high as 80% after one hour. A simple procedure is described in the paper to circumvent this problem allowing reproducible and reliable results to be obtained. A single reagent assay was developed by incorporating phenolphthalein in Na2CO3 solution. This resulted in stable absorbance readings and increased the linearity of assay up to 200μg/ml of β‐cyclodextrin.Keywords
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