In vivo dynamics of human cord blood-derived CD34− SCID-repopulating cells using intra-bone marrow injection

Authors:

TKT Kimura
T Kimura

1 October 2009

journal article
research article
Published by Springer Nature in Leukemia

Vol. 24 (1) , 162-168
DOI:10.1038/leu.2009.206
PMID:19798093

Abstract

The identification of human CD34-negative (CD34⁻) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in the human HSC compartment. This study investigated the long-term repopulating capacity and redistribution kinetics of human cord blood-derived CD34⁻ severe combined immunodeficiency (SCID)-repopulating cells (SRCs) and compared them with those of CD34⁺CD38⁺ and CD34⁺CD38⁻ SRCs using the intra-bone marrow injection (IBMI) to clarify the characteristics of CD34⁻ SRCs. On the basis of the limiting dilution analyses data, estimated numbers of CD34⁺CD38⁺, CD34⁺CD38⁻, and CD34⁻ SRCs were transplanted to NOD/SCID mice by IBMI. The human cell repopulation at the site of injection and the other bones were serially investigated. Interestingly, CD34⁺CD38⁺, CD34⁺CD38⁻, and CD34⁻ SRCs began to migrate to other bones 2 and 5 weeks after the transplantation, respectively. Accordingly, the initiation of migration seemed to differ between the CD34⁺ and CD34⁻ SRCs. In addition, CD34⁺CD38⁺ SRCs only sustained a short-term repopulation. However, both CD34⁺CD38⁻ and CD34⁻ SRCs had longer-term repopulation capacity. Taken together, these findings showed that CD34⁻ SRCs show different in vivo kinetics, thus suggesting that the identified CD34⁻ SRCs are a distinct class of primitive HSCs in comparison to the CD34⁺CD38⁺ and CD34⁺CD38⁻ SRCs.

Keywords

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