A YEAST ARTIFICIAL CHROMOSOME TELOMERE CLONE SPANNING A POSSIBLE LOCATION OF THE HUNTINGTON DISEASE GENE

Authors:
  • MMME MACDONALD
    ME MACDONALD
  • SBS BAXENDALE
    S BAXENDALE
  • ZSZ SEDLACEK
    Z SEDLACEK
  • SYS YOUNGMAN
    S YOUNGMAN
  • DRD ROMANO
    D ROMANO
  • WWWL WHALEY
    WL WHALEY
  • BABA ALLITTO
    BA ALLITTO
  • APA POUSTKA
    A POUSTKA
  • JGJF GUSELLA
    JF GUSELLA
  • HLH LEHRACH
    H LEHRACH
Google Scholar
  • 1 April 1990
    • journal article
    • research article
Abstract
The Huntington disease (HD) gene has been mapped to the most distal subband of chromosome 4p. Analysis of recombination events has not provided an unequivocal location of the HD gene, but it indicates a position very close to the telomere as one possibility. We have constructed a yeast artificial chromosome (YAC) vector (containing a rare-cutter polylinker) for the cloning of mammalian telomeres, used it to prepare a BssHII-telomere library with DNA from an individual homozygous for HD, and have identified a 115-kb clone containing the telomere of 4p. One probable recombinant would confine the telomeric candidate location for the gene to the region covered by the YAC, which makes it possible that the clone described here contains the HD locus in its mutant form.

This publication has 46 references indexed in Scilit: