Separation of Viable from Non-Viable Tumor Cells Using Ficoll-Hypaque Density Solution

Authors:
  • GMGiora M. Mavligit
    Giora M. Mavligit
  • JGJordan U. Gutterman
    Jordan U. Gutterman
  • EHEvan M. Harsh
    Evan M. Harsh
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Abstract
Separation of viable from non-viable human tumor cells was performed by differential flotation on Ficoll-Hypaque specific density solution. A significant purification of viable cell population was achieved, although the total yield was limited. Because viable tumor cells are more active imunologically than non-viable cells, in terms of stimulating blastogenesis among autologous lymphocytes, detection of tumor immunity and recognition in human solid tumors may improve.