A Reference System in Crossed Radioimmunoelectrophoresis

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Abstract
A reference system for crossed radioimmunoelectrophoresis (CRIE) has been developed. The system takes into account the variable quality of the 125I‐labeled anti‐IgE and permits a direct comparison of the results from one experiment to another. Simultaneously with the preparation of the CRIE slides, a standard series of small paper discs are prepared containing varying, defined amounts of 125I‐labeled anti‐IgE. These paper discs are laid out for autoradiography together with the CRIE slides. After short periods of exposure, only paper discs with large amounts of isotope are visible on the X‐ray films. Longer exposure times permit paper discs with smaller amounts of isotope to be visible. Each precipitate is assigned to a CRIE class depending on the number of paper discs visible when the precipitate is detected for the first time.

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