Hydrophilic interaction liquid chromatography/electrospray mass spectrometry determination of acyclovir in pregnant rat plasma and tissues

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Abstract
Reversed‐phase chromatography is the most common means of separation for small drug molecules. However, polar drugs may suffer from poor retention and peak shape in reversed‐phase high‐performance liquid chromatography (RP‐HPLC). Hydrophilic interaction liquid chromatography (HILIC) provides a viable alternative to RP‐HPLC and is an excellent way to separate polar compounds. This paper describes a HILIC/ESI‐MS/MS assay for the determination of acyclovir from rat plasma, amniotic fluid, placental tissue, and fetal tissue. The isocratic separation utilizes an underivatized silica column with an acetonitrile/formate buffer mobile phase (80:20). The method is validated over a range of 50 ng/mL to 50 µg/mL with % error and % relative standard deviation of 84% for acyclovir). This assay can be applied to the pharmacokinetic study of the placental transfer of acyclovir. Copyright © 2002 John Wiley & Sons, Ltd.

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