The Binding of Vitamin B12 Added in Vitro and in Vivo to Normal Human Serum Proteins Separated on Ion Exchange Cellulose

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Abstract
The binding of vitamin B12 (as cyanocobalamin) added to human serum in vitro and in vivo was studied in three normal subjects. Chromatographic separation on DEAE cellulose demonstrated that added vitamin B12 was bound to a limited degree chiefly to a protein distinct from that to which native vitamin B12 is complexed