Advantages of Epoxy Resin as a Mounting Medium for Light Microscopy
Author:
- 1 January 1971
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 46 (6) , 315-316
Abstract
The need for very durable mounting is especially felt in the teaching of parasitology and mycology; otherwise, the availability of microscope slides may depend on the use of fresh specimens. Resinous mounts and those in aqueous media sealed with fingernail lacquer, paraffin or asphalt do not preserve specimens satisfactorily. Polyvinylpyrrolidone (pvp), a water-soluble mounting medium described by Burstone (1962), cannot be applied directly for mounting of insects and certain other parasites which have water-repelling integuments; moreover, pvp bleaches eosin. Grimley et al. (1965) prepared large epoxy sections of tissues from which areas for electron microscopy could be selected. This procedure however is designed for electron microscopic techniques whereas the present paper describes a direct epoxy mounting method to produce permanent mounts for light microscopy.Keywords
This publication has 2 references indexed in Scilit:
- Preparation of Large Epoxy Sections for Light Microscopy as an Adjunct to Fine-Structure StudiesStain Technology, 1965
- Notes on TechnicStain Technology, 1963