MiR‐137 knockdown promotes the osteogenic differentiation of human adipose‐derived stem cells via the LSD1/BMP2/SMAD4 signaling network

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Abstract
MicroRNAs are a group of endogenous regulators that participate in several cellular physiological processes. However, the role of miR‐137 in the osteogenic differentiation of human adipose‐derived stem cells (hASCs) has not been reported. This study verified a general downward trend in miR‐137 expression during the osteogenic differentiation of hASCs. MiR‐137 knockdown promoted the osteogenesis of hASCs in vitro and in vivo. Mechanistically, inhibition of miR‐137 activated the bone morphogenetic protein 2 (BMP2)‐mothers against the decapentaplegic homolog 4 (SMAD4) pathway, whereas repressed lysine‐specific histone demethylase 1 (LSD1), which was confirmed as a negative regulator of osteogenesis in our previous studies. Furthermore, LSD1 knockdown enhanced the expression of BMP2 and SMAD4, suggesting the coordination of LSD1 in the osteogenic regulation of miR‐137. This study indicated that miR‐137 negatively regulated the osteogenic differentiation of hASCs via the LSD1/BMP2/SMAD4 signaling network, revealing a new potential therapeutic target of hASC‐based bone tissue engineering.
Funding Information
  • National Natural Science Foundation of China (81700937)

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