Sequence Note: Identification of All HIV Type 1 Group M Subtypes in Senegal, a Country with Low and Stable Seroprevalence

Authors:
  • CMCeline Montavon
    Celine Montavon
  • MFMame Awa Faye
    Mame Awa Faye
  • PMPape Mandoumb
    Pape Mandoumb
  • EAEacute
    Eacute
  • PSPapa Salif Sow
    Papa Salif Sow
  • INIbra Ndoye
    Ibra Ndoye
  • AGAissatou Gaye-Diallo
    Aissatou Gaye-Diallo
  • MPMartine Peeters
    Martine Peeters
  • SMSouleymane Mboup
    Souleymane Mboup
Publisher website
Google Scholar
Abstract
A total of 343 HIV-1-positive samples obtained between June 1996 and March 1999 was genetically characterized in the envelope region by HMA and/or sequencing. The env subtype distribution was as follows: 290 (84.6%) A, 22 (6.5%) B, 16 (4.7%) C, 8 (2.5%) D, 1 (0.03%) E, 1 (0.03%) F1, 4 (1.2%) G, and 1 (0.03%) H. For 77 samples the p24 region from the gag gene was also sequenced, and for 9 (11.6%) the subtypes between env and gag were different. Phylogenetic tree analysis showed the predominance of AG-IBNG-like viruses among gag and env subtype A sequences. HMA is relatively simple and requires less sophisticated technical facilities compared with sequencing, and in Senegal 323 (94.2%) of the 343 samples could be identified by this technique. However, in the actual configuration of the assay, discrimination between the recombinant AGIBNG-like recombinant viruses, which are predominant in Senegal, and the nonrecombinant subtype A viruses is not possible.

This publication has 17 references indexed in Scilit: