On the inscrutable role of Inscuteable: structural basis and functional implications for the competitive binding of NuMA and Inscuteable to LGN
Open Access
- 1 August 2012
- journal article
- review article
- Published by The Royal Society in Open Biology
- Vol. 2 (8) , 120102
Abstract
Alignment of the mitotic spindle to the cellular polarity axis is a prerequisite for asymmetric cell divisions. The protein network coordinating the spindle position with cortical polarity includes the molecular machinery pulling on astral microtubules, which is assembled on conserved NuMA:LGN:Gαi complexes, the polarity proteins Par3:Par6:aPKC and an adaptor molecule known as Inscuteable (Insc). To date, all these components were assumed to enter a macromolecular complex localized at polarity sites in mitosis. However, recent structural studies revealed the Insc and NuMA are mutually exclusive interactors of LGN, implying that the molecular mechanism of spindle coupling to polarity is more sophisticated than has been believed to date.Keywords
This publication has 53 references indexed in Scilit:
- Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientationNature Cell Biology, 2012
- Mouse Inscuteable Induces Apical-Basal Spindle Orientation to Facilitate Intermediate Progenitor Generation in the Developing NeocortexNeuron, 2011
- An Ana2/Ctp/Mud Complex Regulates Spindle Orientation in Drosophila NeuroblastsDevelopmental Cell, 2011
- LGN/mInsc and LGN/NuMA Complex Structures Suggest Distinct Functions in Asymmetric Cell Division for the Par3/mInsc/LGN and Gαi/LGN/NuMA PathwaysMolecular Cell, 2011
- Robust Spindle Alignment in Drosophila Neuroblasts by Ultrasensitive Activation of PinsMolecular Cell, 2011
- Asymmetric cell divisions promote Notch-dependent epidermal differentiationNature, 2011
- Cell polarity and spindle orientation in the distal epithelium of embryonic lungDevelopmental Dynamics, 2011
- Par3 Controls Epithelial Spindle Orientation by aPKC-Mediated Phosphorylation of Apical PinsCurrent Biology, 2010
- Identification of an Aurora-A/PinsLINKER/ Dlg Spindle Orientation Pathway using Induced Cell Polarity in S2 CellsCell, 2009
- Adherens junction domains are split by asymmetric division of embryonic neural stem cellsEMBO Reports, 2009