SDS—PAGE strongly overestimates the molecular masses of the neurofilament proteins
Authors:
- Eckhard KaufmannEKEckhard Kaufmann
- Norbert GeislerNGNorbert Geisler
- Klaus WeberKWKlaus Weber
- 7 May 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 170 (1) , 81-84
Abstract
Direct molecular mass determination of the three porcine neurofilament proteins (H, M and L) was performed in 6 M guanidine—HCl using analytical gel filtration and sedimentation equilibrium centrifugation. The results show that SDS—PAGE strongly overestimates the values of the ‘higher molecular mass’ components H and M. This discrepancy stems from the carboxyterminal extensions known to have unusual amino acid composition.Keywords
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