An electrochemical aptasensing platform for carbohydrate antigen 125 based on the use of flower-like gold nanostructures and target-triggered strand displacement amplification

Authors:
Publisher website
Google Scholar
Abstract
An electrochemical aptasensing method is described for the determination of the biomarker CA125. It combines aptamer recognition and target-triggered strand displacement amplification. Flower like gold nanostructures were electrodeposited on a screen-printed carbon electrode to increase the sensor surface, to assemble more toehold-containing hairpin probe 1 (Hp1), and to improve the accessibility for DNA strands. Under the optimal conditions, this assay has a linear response in the 0.05 to 50 ng•mL−1 CA125 concentration range, with a low detection limit of 5.0 pg•mL−1. This method is specific and stable. It was successfully applied to the detection of CA125 in spiked biological samples, with recoveries between 82.5% and 104.1%.
Funding Information
  • Science and Technology Key Program of Shiyan (18Y101, 18Y102, 18Y104)
  • National Natural Science Foundation of China (81872509)
  • Hubei Provincial Technology Innovation Project (2017ACA176)
  • Free Exploration Project of Hubei University of Medicine (FDFR201804)
  • Key Discipline Project of Hubei University of Medicine and the Foundation of Health Commission of Hubei Province (WJ2019M054)
  • Research Project of Traditional Chinese Medicine of the Foundation of Health Commission of Hubei Province (ZY2019M034)
  • Open Project of Hubei Key Laboratory of Wudang Local Chinese Medicine Research (WDCM2018004)

This publication has 35 references indexed in Scilit: