OVEREXPRESSION CONFERS AN ONCOGENIC POTENTIAL UPON THE EPH GENE
Authors:
- H HIRAIHHH HIRAI
- F TAKAKUFTF TAKAKU
- 1 March 1990
- journal article
- research article
- Vol. 5 (3) , 445-447
Abstract
The eph gene encodes a putative receptor tyrosine kinase for an as yet unknown ligand. Some human cancer cells have been found to overexpress eph mRNAs without gene amplification. We show here that NIH3T3 cells acquire tumorigenic ability in nude mice and make colonies in soft agar with a viral LTR (Long Terminal Repeat)-driven artifical expression of the eph gene to a high level. This result supports the alleged contribution of overexpressed receptor tyrosine kinases to cell transformation.Keywords
This publication has 19 references indexed in Scilit:
- Amplification and overexpression of the met gene in spontaneously transformed NIH3T3 mouse fibroblasts.The EMBO Journal, 1986
- Characterization of an activated human ros gene.Molecular and Cellular Biology, 1986
- A retrovirus vector expressing the putative mammary oncogene int-1 causes partial transformation of a mammary epithelial cell lineCell, 1986
- Mechanism of met oncogene activationCell, 1986
- Multiple independent activations of the neu oncogene by a point mutation altering the transmembrane domain of p185Cell, 1986
- Isolation of hybridomas expressing a specific heavy chain variable region gene segment by using a screening technique that detects mRNA sequences in whole cell lysates.Proceedings of the National Academy of Sciences, 1984
- New Method for Detecting Cellular Transforming GenesScience, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virusJournal of Virology, 1980
- Antibodies specific for the carboxy- and amino-terminal regions of simian virus 40 large tumor antigen.Proceedings of the National Academy of Sciences, 1980