An octamer oligonucleotide upstream of a TATA motif is sufficient for lymphoid-specific promoter activity
Authors:
- Thomas WirthTWThomas Wirth
- Louis StaudtLSLouis Staudt
- David BaltimoreDBDavid Baltimore
- 1 September 1987
- journal article
- Published by Springer Nature in Nature
- Vol. 329 (6135) , 174-178
Abstract
The octamer sequence ATGCAAAT or its inverse complement ATTTGCAT is well-conserved in all immunoglobulin gene promoters and has been implicated in promoter function by deletion analysis. Although immunoglobulin promoters are tissue-specific, the octamer is also a functional element in non-tissue-specific upstream regions--like those controlling U1 and U2 small nuclear RNA and histone H2B genes--where it is associated with additional canonical elements. Specific interactions occur between the octamer motif and both lymphoid-specific and ubiquitous proteins. By using a synthetic octamer oligonucleotide inserted upstream of the beta-globin TATA box we show here that the octamer element by itself is sufficient for directing lymphocyte-specific RNA synthesis when within 70 base pairs of the start site of transcription. We also demonstrate that mutations in any position of the conserved motif interfere with this function.Keywords
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