A novel sandwich-type electrochemical aptasensor for sensitive detection of kanamycin based on GR–PANI and PAMAM–Au nanocomposites

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Abstract
In this work, we report a novel sandwich-type electrochemical aptasensor for sensitive and selective detection of antibiotic kanamycin. This aptasensor was constructed by assembling graphene–polyaniline (GR–PANI) and polyamidoamine dendrimer–Au nanoparticle (PAMAM–Au) nanocomposites on the surfaces of a glass carbon electrode, which as electrode materials exhibited excellent charge-transport property and loading capacity of biomolecules. Followed by the sandwich-type specific identification, kanamycin detection was realized by outputting a redox current from electro-reduction reaction of H2O2 catalyzed by HRP. It was demonstrated that the developed strategy had a wide linear response range from 5 × 10−6 to 4 × 10−2 μg mL−1 and a low detection limit of 4.6 × 10−6 μg mL−1. Additionally, this aptasensor had the advantages in its low cost, high sensitivity, and superb specificity. Thus, it might provide a useful tool for kanamycin determination and related food safety analysis and clinical diagnosis.

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