Genome-Wide Identification and Drought Stress-Responsive Expression Profiling of the FAD Gene Family in Pear

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Abstract
Fatty acid desaturase (FAD) is a rate-limiting enzyme catalyzing the biosynthesis of unsaturated fatty acids (UFAs) and participates in key physiological processes such as plant growth and development, fruit ripening, and stress responses by regulating membrane lipid composition. Using pear genome data, this study systematically identified FAD gene family members through bioinformatic analysis and characterized their drought-responsive expression patterns. Results revealed that 34 FAD family members were identified in pear, unevenly distributed across 12 chromosomes and classified into six subfamilies. Members within the same subfamily exhibited similar conserved domains and gene structures. Promoter element analysis demonstrated that pear FAD promoters contain cis-acting elements associated with plant growth and development, hormone responses, and abiotic stress responses. qRT-PCR expression profiling showed that PbrFAD23 and PbrFAD30 were significantly upregulated during the early stages of drought stress, followed by suppressed expression levels, suggesting their potential crucial regulatory roles in the initial drought response. Genome-wide identification of 34 PbrFAD family members highlighted that PbrFAD23 and PbrFAD30, with marked upregulation under early drought stress, exhibit prominent drought responsiveness. This study provides valuable resistance gene resources for molecular breeding of stress-tolerant pear varieties and establishes a theoretical foundation for functional characterization of key drought-resistant candidate genes in pear.
Funding Information
  • Hebei Agriculture Research System (HBCT2024170204, 21422012321, C2022402006)
  • Handan Science and Technology Plan Project (HBCT2024170204, 21422012321, C2022402006)
  • Hebei Natural Science Foundation General Project (HBCT2024170204, 21422012321, C2022402006)

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