Proteomic Analysis of Human Immunodeficiency Virus Using Liquid Chromatography/Tandem Mass Spectrometry Effectively Distinguishes Specific Incorporated Host Proteins
Authors:
- 11 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 5 (3) , 530-538
Abstract
A major challenge to studying virus-incorporated host proteins is the fact that they are not encoded by the viral genome. We used Liquid Chromatography/Tandem Mass Spectrometry (LC−MS/MS) on whole virions to obtain a snapshot of the HIV-1 proteome. We identified known viral and host-cellular proteins and also identified novel components of HIV-1 and confirm these by traditional biochemical methods. Our comparison of wild-type and mutant viruses demonstrates that LC−MS/MS has the specificity to distinguish the presence/absence of a single host protein in intact virions. Keywords: proteomics • HIV • tandem mass spectrometry • liquid chromatographyKeywords
This publication has 47 references indexed in Scilit:
- Human Immunodeficiency Virus Type 1-Induced Macrophage Gene Expression Includes the p21 Gene, a Target for Viral RegulationJournal of Virology, 2005
- Covalent Modification of Human Immunodeficiency Virus Type 1 p6 by SUMO-1Journal of Virology, 2005
- Potential for False Positive Identifications from Large Databases through Tandem Mass SpectrometryJournal of Proteome Research, 2004
- The Identification of an Adenovirus Receptor by Using Affinity Capture and Mass SpectrometryChemBioChem, 2004
- HLA-DR, ICAM-1, CD40, CD40L, and CD86 are incorporated to a similar degree into clinical human immunodeficiency virus type 1 variants expanded in natural reservoirs such as peripheral blood mononuclear cells and human lymphoid tissue cultured ex vivoClinical Immunology, 2004
- A proteomic view of the Plasmodium falciparum life cycleNature, 2002
- Envelope Glycoprotein Incorporation, Not Shedding of Surface Envelope Glycoprotein (gp120/SU), Is the Primary Determinant of SU Content of Purified Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency VirusJournal of Virology, 2002
- Cyclophilin A Plays Distinct Roles in Human Immunodeficiency Virus Type 1 Entry and Postentry Events, as Revealed by SpinoculationJournal of Virology, 2002
- Characterization of HIV Replication Complexes Early after Cell-to-Cell InfectionAIDS Research and Human Retroviruses, 1993
- Cytomegalovirus in Urine Specimens Has Host beta2 Microglobulin Bound to the Viral Envelope: A Mechanism of Evading the Host Immune Response?Journal of General Virology, 1987