COMPARISON OF VIABILITY OF STRAINS OF NEUROSPORA AFTER STORAGE IN LIQUID NITROGEN
Author:
- A. M. WellmanAWA. M. Wellman
- 1 June 1967
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 13 (6) , 665-672
Abstract
The survival of mutant and wild type macroconidia of Neurospora was compared after the conida, in the "air dry" state, had been frozen rapidly to −196 °C in liquid nitrogen and thawed rapidly. The 16 isolates of Neurospora were compared after 3 months' storage in liquid nitrogen with 7-day-old control cultures. Eleven of the 16 isolates showed no significant difference from 7-day-old controls in the pattern of germination after 8 hours' incubation. In the remainder, those spores germinating did so at a rate comparable to that of the controls, but the final proportion germinating was 5.0–19.0% lower.Keywords
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