The stereoselective enzymatic synthesis of 9-β-d-2′-deoxyribofuranosyl 1-deazapurine
Authors:
- D. BetbederDBD. Betbeder
- D.W. HutchinsonDHD.W. Hutchinson
- A.O'L. RichardsARA.O'L. Richards
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (11) , 4217-4222
Abstract
The transfer of 2-deoxyribose from thymidine to 1-deazapurine which is catalysed by N-deoxyribosyl transferases from Lactobacillus leichmanii occurs in high yield. This is a very stereoselective process and only one product, 9-beta-D-2'-deoxyribofuranosyl 1-deazapurine, is formed. Nmr spectroscopy, and in particular, nuclear Overhauser enhancement experiments, confirm that the 2-deoxyribose moiety is bound to N-9 rather than N-7 and that the glycosidic link has the beta-configuration.Keywords
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