Image patterned molecular delivery into live cells using gold particle coated substrates
Authors:
Open Access
- 7 January 2010
- journal article
- Published by Optica Publishing Group in Optics Express
- Vol. 18 (2) , 938-946
Abstract
An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated.Keywords
This publication has 16 references indexed in Scilit:
- Pulsed-laser-induced dewetting in nanoscopic metal films: Theory and experimentsPhysical Review B, 2007
- Nanocrystalline barium titanate films on flexible plastic substrates via pulsed laser annealingApplied Physics Letters, 2006
- Selective laser nano‐thermolysis of human leukemia cells with microbubbles generated around clusters of gold nanoparticlesLasers in Surgery and Medicine, 2006
- Excitation of nanoscale vapor bubbles at the surface of gold nanoparticles in waterThe Journal of Chemical Physics, 2006
- Calculated Absorption and Scattering Properties of Gold Nanoparticles of Different Size, Shape, and Composition: Applications in Biological Imaging and BiomedicineThe Journal of Physical Chemistry B, 2006
- Optoinjection for efficient targeted delivery of a broad range of compounds and macromolecules into diverse cell typesJournal of Biomedical Optics, 2006
- Pulsed-laser-induced nanoscale island formation in thin metal-on-oxide filmsPhysical Review B, 2005
- Elevation of plasma membrane permeability by laser irradiation of selectively bound nanoparticlesJournal of Biomedical Optics, 2005
- Optical Constants of the Noble MetalsPhysical Review B, 1972
- Heat flow in an infinite medium heated by a sphereBritish Journal of Applied Physics, 1952