The IBEX knowledge-base a community resource enabling adoption and development of immunofluorescence imaging methods

Authors:
Open Access
Publisher website
Google Scholar
Abstract
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, such as primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.
Funding Information
  • National Institutes of Health (HHSN316201300006W/75N93022F00001)
  • National Institutes of Health (OT2OD033756)
  • National Institutes of Health (U24CA268108)
  • National Institutes of Health (U24DK135157)
  • National Institutes of Health (U2CDK114886)
  • Wellcome Trust (10.35802/224586)
  • Wellcome Trust (10.35802/220268)
  • National Institutes of Health (R01AR077019)
  • National Institutes of Health (R01AI134713)
  • National Institutes of Health (75N93019C00070)
  • Damon Runyon Cancer Research Foundation (DRG 2409-20)
  • European Research Council (771883)
  • European Research Council (819329-STEP2)
  • Biotechnology and Biological Sciences Research Council (BB/S010386/1)
  • Wellcome Trust (222096/Z/20/Z)
  • National Institutes of Health (K99/R00 4R00GM147841-02)
  • Canadian Institutes of Health Research (170793)
  • NIHR Cambridge Biomedical Research Centre (NIHR203312)
  • NIHR Blood and Transplant Research Unit in Organ Donation and Transplantation (NIHR203332)
  • CNPq, FAPEMIG, FAPESP (#2021/00408–6)
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP fellowship 2023/01697-7)
  • National Polytechnic Institute (IPN) of Mexico (DRI/DII/0445/2024)
  • National Council of Humanities, Science and Technology (B210344)
  • National Council of Humanities, Science and Technology (CVU: 10008)

This publication has 51 references indexed in Scilit: