Visualizing Intercellular Tensile Forces by DNA-Based Membrane Molecular Probes

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Abstract
Mechanical forces play critical roles in collective cell behaviors such as cell migration, proliferation, and differentiation. Extensive efforts have been made to measure forces between cells and extracellular matrices. However, force studies at cell–cell junctions remain a challenge. Herein, we reported a novel strategy to construct membrane DNA tension probes to visualize tensile forces at cell junctions. These lipid-modified probes can self-assemble onto cell membranes with high efficiency and stability. Upon experiencing tensile forces generated by neighboring cells, unfolding of the probes leads to a large increase in the fluorescence intensity. Compatible with readily accessible fluorescence microscopes, these easy-to-use membrane DNA tension probes can be broadly used to measure intercellular tensile forces.
Funding Information
  • Division of Civil, Mechanical and Manufacturing Innovation (CMMI 1662835)
  • University of Massachusetts Amherst
  • Institute for Applied Life Sciences, University of Massachusetts Amherst

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