KINETICS OF STACKING INTERACTIONS IN FLAVIN ADENINE DINUCLEOTIDE FROM TIME‐RESOLVED FLAVIN FLUORESCENCE
Author:
- 1 December 1984
- journal article
- research article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 40 (6) , 703-706
Abstract
The time dependence of the fluorescence of flavin adenine dinucleotide (FAD) was measured with a subnanosecond‐resolving fluorometer. In contrast to the fluorescence decay of FMN, the decay of FAD was proved to be nonexponential. The time‐dependent fluorescence of FAD can be interpreted by assuming an equilibrium between closed and open conformers in the ground state. The rate constant for folding in the excited state and the fluorescence lifetime of the intramolecular complex could be evaluated from analysis of the observed fluorescence decay. The results on FAD were compared to those on NADH obtained earlier.Keywords
This publication has 20 references indexed in Scilit:
- SPECTROSCOPIC AND DYNAMIC CHARACTERIZATION OF FMN IN REVERSED MICELLES ENTRAPPED WATER POOLSPhotochemistry and Photobiology, 1984
- THE FLUORESCENCE DECAY OF REDUCED NICOTINAMIDES IN AQUEOUS SOLUTION AFTER EXCITATION WITH A UV‐MODE LOCKED Ar ION LASERPhotochemistry and Photobiology, 1981
- Crystal structure of p-hydroxybenzoate hydroxylaseJournal of Molecular Biology, 1979
- Absorption and Fluorescence Studies on Neutral and Cationic IsoalloxazinesHelvetica Chimica Acta, 1979
- Effect of pressure upon the fluorescence of various flavodoxinsBiochemistry, 1977
- Time resolved fluorescence of flavin adenine dinucleotideFEBS Letters, 1974
- Conformation in the excited state of two tryptophanyl diketopiperazinesJournal of the American Chemical Society, 1974
- A 13C Nuclear‐Magnetic‐Resonance Study of the Enzyme Cofactor Flavin‐Adenine DinucleotideEuropean Journal of Biochemistry, 1972
- Excited-state proton transfer as a biological probe. Determination of rate constants by means of nanosecond fluorometryBiochemistry, 1972
- Time resolved electronic spectroscopy. I. Bimolecular rates for excimer formationAustralian Journal of Chemistry, 1969