This work focuses on the preparation and characterization of chitosan (CS) at different degrees of deacetylation (CS 74%, CS 86%, CS 90% and CS 95%) in order to use them as adsorbents to remove fluoride ions. The characterization of the materials was carried out by X-ray diffraction, Infrared spectroscopy and determination the degrees of deacetylation (DD) by conductometric titration. The influence of various physico-chemical parameters such as adsorbent dose, contact time, pH, initial fluoride concentration and temperature were studied. The adsorption kinetics was studied using pseudo-first order and pseudo-second order kinetic equations. The adsorption isotherm was investigated by Langmuir and Freundlich models.