EFFECT OF THE LOCAL ADMINISTRATION OF PROTEOLYTIC ENZYMES ON THE RUPTURE OF THE OVARIAN FOLLICLE IN THE DOMESTIC FOWL, GALLUS DOMESTICUS

Authors:
  • SNS. Nakajo
    S. Nakajo
  • AZA. H. Zakaria
    A. H. Zakaria
  • KIK. Imai
    K. Imai
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Abstract
Summary.: An attempt was made to demonstrate whether proteases administered locally to the ovarian follicle of the hen would result in its rupture. Using 'ovulation-suppressed' pullets prepared with a daily injection of PMSG, a large ovarian follicle was brought to the surface and treated with the enzyme solutions. When 'collagenase' and nonspecific proteases were injected into the follicle wall or applied on a small piece of filter-paper placed on the follicular surface, rupture was induced in almost all the follicles within 2 to 3 hr, whereas no rupture occurred in the follicles treated with hyaluronidase, lysozyme or saline solution. It is suggested that proteolytic enzymes might be involved in the mechanism of normal rupture of the ovarian follicle in the hen.