Proteomic analysis of soybean defense response induced by cotton worm (prodenia litura, fabricius) feeding
Open Access
- 8 March 2012
- journal article
- Published by Springer Nature in Proteome Science
- Vol. 10 (1) , 16
Abstract
Background: Cotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding. Results: Ten protein spots that are changed in abundance in response to cotton worm feeding were identified by Two-dimensional gel electrophoresis (2-DE). A total of 11 unique proteins from these spots were identified by MALDI-TOF MS. The mRNA and protein relative expression levels of most changed proteins were up-regulated. These proteins were mainly involved in physiological processes, including active oxygen removal, defense signal transduction, and metabolism regulation. Conclusion: This is the first proteomic analysis of the soybean defense response induced by cotton worm. The differentially expressed proteins could work together to play a major role in the induced defense response. PAL and SAMS were up-regulated at both the protein and mRNA levels. These genes can be strongest candidates for further functional research.Keywords
This publication has 73 references indexed in Scilit:
- Proteolysis of a Negative Regulator of Innate Immunity Is Dependent on Resistance Genes in Tomato and Nicotiana benthamiana and Induced by Multiple Bacterial EffectorsThe Plant Cell, 2009
- Methyl Salicylate Production and Jasmonate Signaling Are Not Essential for Systemic Acquired Resistance inArabidopsisThe Plant Cell, 2009
- Within-plant signaling by volatiles leads to induction and priming of an indirect plant defense in natureProceedings of the National Academy of Sciences, 2007
- Herbivory Rapidly Activates MAPK Signaling in Attacked and Unattacked Leaf Regions but Not between Leaves ofNicotiana attenuataThe Plant Cell, 2007
- Correlation of mRNA Expression and Protein Abundance Affected by Multiple Sequence Features Related to Translational Efficiency in Desulfovibrio vulgaris: A Quantitative AnalysisGenetics, 2006
- Herbivore-Induced Resistance against Microbial Pathogens in ArabidopsisPlant Physiology, 2006
- A proteomic analysis of cold stress responses in rice seedlingsProteomics, 2005
- Defensive Function of Herbivore-Induced Plant Volatile Emissions in NatureScience, 2001
- The timing of induced resistance and induced susceptibility in the soybean-Mexican bean beetle systemOecologia, 1998
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976