A microsystem for the extraction and in-vitro translation of mouse embryo mRNA

Authors:
  • PBP. R. Braude
    P. R. Braude
  • HPH. R. B. Pelham
    H. R. B. Pelham
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Abstract
Summary. A micromethod is described for the extraction of mRNA from mouse ova and its translation in a message-dependent rabbit reticulocyte lysate system. Using this technique it has been possible, by SDS polyacrylamide gel fluorography, to visualize the products from the equivalent of 12·5 eggs.