Structure Validation of G‐Rich RNAs in Noncoding Regions of the Human Genome
Open Access
- 14 January 2020
- journal article
- research article
- Published by Wiley in ChemBioChem
- Vol. 21 (11) , 1656-1663
Abstract
We present the rapid biophysical characterization of six previously reported putative G‐quadruplex‐forming RNAs from the 5′‐untranslated region (5′‐UTR) of silvestrol‐sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]2[CGG]2C—folds into a single well‐defined G‐quadruplex structure. Sequences with longer poly‐G strands form unspecific aggregates, whereas CGG‐repeat‐containing sequences exhibit a temperature‐dependent equilibrium between a hairpin and a G‐quadruplex structure. The applied experimental strategy is fast and provides robust readout for G‐quadruplex‐forming capacities of RNA oligomers.Keywords
Funding Information
- Deutsche Forschungsgemeinschaft (SFB902)
- Hessisches Ministerium für Wissenschaft und Kunst (BMRZ)
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