High-Resolution Melting Analysis of the spa Repeat Region of Staphylococcus aureus
- 1 February 2008
- journal article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 54 (2) , 432-436
Abstract
Background: The staphylococcal protein A (spa) locus of Staphylococcus aureus contains a complex repeat structure and is commonly used for single-locus sequence-based genotyping. The real-time PCR platform supports genotyping methods that are single step and closed tube and potentially can be carried out simultaneously with diagnosis. We describe here a method for genotyping S. aureus using high-resolution melting (HRM) analysis of the spa polymorphic region X.Keywords
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