Purification of glycoprotein gD of herpes simplex virus types 1 and 2 by use of monoclonal antibody
Authors:
- R J EisenbergRER J Eisenberg
- M Ponce de LeonMLM Ponce de Leon
- L PereiraLPL Pereira
- D LongDLD Long
- G H CohenGCG H Cohen
- 1 March 1982
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 41 (3) , 1099-1104
Abstract
Glycoproteins gD-1 and gD-2 of herpes simplex virus types 1 and 2, respectively, were purified on an immunoadsorbent consisting of the type-common monoclonal antibody HD-1 linked to Sepharose. Each glycoprotein was of sufficient purity, quantity, and biological activity to be used for immunological and biochemical studies. Each glycoprotein induced high titers of type-common monospecific neutralizing antibody in mice. Amino aicd analysis indicated that gD-1 and gD-2 had similar though not identical amino acid compositions.Keywords
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