Folding transition into a loosely collapsed state in plasmid DNA as revealed by single‐molecule observation
Authors:
- Tsutomu HamadaTHTsutomu Hamada
- Koji KuboKKKoji Kubo
- Ayako YamadaAYAyako Yamada
- Tsunao KishidaTKTsunao Kishida
- Osam MazdaOMOsam Mazda
- Kenichi YoshikawaKenichi Yoshikawa
- 12 May 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (14) , 3095-3099
Abstract
The conformational transition of a plasmid DNA, pGEG.GL3 (12.5 kbp, circular), induced by spermine(4+) was studied through the observation of individual DNA by fluorescence microscopy. We deduced the change in the hydrodynamic radius R H from an analysis of the Brownian motion of single DNA molecules. R H decreases in a continuous manner with an increase in spermine(4+), in contrast to the large discrete on/off change for long linear DNA. Just after the transition to the collapsed state, a small number of DNA molecules tend to form an assembly, which disperses in the bulk solution without precipitation.Keywords
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