Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy
Authors:
- 15 March 2010
- journal article
- conference paper
- Published by AIP Publishing in Applied Physics Letters
- Vol. 96 (11) , 113701
Abstract
Heme proteins, such as hemoglobins and cytochromes, play important roles in various biological processes. Here we employ the two-photon excited photothermal effect as a contrast mechanism to map heme proteins distribution. Particularly, both a thermal lens scheme and a high-frequency modulation are utilized to enhance the signal-to-noise ratio. We demonstrate label-free imaging of individual red blood cells, subcellular distribution of cytochromes in live mammalian cells, and the microvascular networks in mouse ear tissue and in a zebrafish gill.Keywords
This publication has 19 references indexed in Scilit:
- Imaging chromophores with undetectable fluorescence by stimulated emission microscopyNature, 2009
- Noninvasive, in vivo imaging of blood-oxygenation dynamics within the mouse brain using photoacoustic microscopyJournal of Biomedical Optics, 2009
- Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering MicroscopyScience, 2008
- Photothermal Lens Detection of Gold Nanoparticles: Theory and ExperimentsApplied Spectroscopy, 2007
- High-resolution imaging of the dynamic tumor cell–vascular interface in transparent zebrafishProceedings of the National Academy of Sciences, 2007
- Large two-photon absorptivity of hemoglobin in the infrared range of 780–880nmThe Journal of Chemical Physics, 2007
- In Vivo Imaging of Mammalian Cochlear Blood Flow Using Fluorescence MicroendoscopyOtology & Neurotology, 2006
- Two-Photon Imaging of Cortical Surface Microvessels Reveals a Robust Redistribution in Blood Flow after Vascular OcclusionPLOS Biology, 2006
- Excited State Dynamics and Heterogeneity of Folded and Unfolded States of CytochromecThe Journal of Physical Chemistry B, 2002
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990