Growth of protozoa in tissue culture. V. Leishmania donovani
Author:
- 1 January 1948
- journal article
- research article
- Published by Oxford University Press (OUP) in Transactions of the Royal Society of Tropical Medicine and Hygiene
- Vol. 41 (4) , 545-554
Abstract
Leishmania donovani has been grown in tissue culture at 37° C., using the technique previously described for the exoerythrocytic forms of P. gallinaceum. The cultures were made from the spleen of infected hamsters, but rabbit serum was substituted for hamster serum in the fluid phase of the medium, because it was more easily obtainable. Vigorous growth of leishmania occurred and growth was maintained as long as 32 days. Infection could easily be transmitted to cultures of clean spleen grown in the same Carrell flask as an infected culture.Keywords
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