Comparison of the selenium level with GSH‐Px activity in the liver of mice treated with 7,12 DMBA

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Abstract
In this study, the relationship between selenium (Se) and glutathione peroxidase (GSHPx) levels was investigated in 7,12‐dimethylbenz(a)anthracene (7,12‐DMBA)‐treated mouse liver. The potential mammary carcinogen 7,12‐DMBA, was injected intraperitoneally (20 mg kg−1 day−1) into 10–12 month old female mice. After 21 days of application the mice were sacrificed and GSHPx and Se levels of liver homogenates were measured. Se and GSHPx levels in 7,12‐DMBA‐treated mice were significantly lower than those of controls (p < 0.05). The control group exhibited 0.9 ± 0.066 U mg−1 protein and 0.86 ± 0.058 p.p.m. levels of GSHPx and Se respectively. The 7,12‐DMBA‐treated group had significantly (p < 0.05) decreased GSHPx and Se levels (0.42 ± 0.062 U mg−1 protein and 0.69 ± 0.034 p.p.m. respectively). The results show a direct relationship between Se and GSHPx activity and a negative correlation between antioxidant capacity and existence of a carcinogen in metabolism. Copyright © 2001 John Wiley & Sons, Ltd.

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