Analysis of Pesticides in Honey by Solid-Phase Extraction and Gas Chromatography−Mass Spectrometry
Authors:
- 26 August 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 52 (19) , 5828-5835
Abstract
An analytical method for the simultaneous determination of 51 pesticides in commercial honeys was developed. Honey (10 g) was dissolved in water/methanol (70:30; 10 mL) and transferred to a C18 column (1 g) preconditioned with acetonitrile and water. Pesticides were subsequently eluted with a hexane/ethyl acetate mixture (50:50) and determined by gas chromatography with electron impact mass spectrometric detection in the selected ion monitoring mode (GC-MS-SIM). Spiked blank samples were used as standards to counteract the matrix effect observed in the chromatographic determination. Pesticides were confirmed by their retention times, their qualifier and target ions, and their qualifier/target abundance ratios. Recovery studies were performed at 0.1, 0.05, and 0.025 μg/g fortification levels for each pesticide, and the recoveries obtained were >86% with relative standard deviations of 0.996. The proposed method was applied to the analysis of pesticides in honey samples, and low levels of a few pesticides (dichlofluanid, ethalfluralin, and triallate) were detected in some samples. Keywords: Multiresidue; honey; pesticides; gas chromatography−mass spectrometry; SPEKeywords
This publication has 21 references indexed in Scilit:
- Assessment of Pesticide Residues in Honey Samples from Portugal and SpainJournal of Agricultural and Food Chemistry, 2003
- Fluvalinate residues in honey by capillary gas chromatography—Electron capture detection—Mass spectrometryChromatographia, 2002
- Study of semi-automated solid-phase extraction for the determination of acaricide residues in honey by liquid chromatographyJournal of Chromatography A, 2001
- A SPME-GC-MS approach for antivarroa and pesticide residues analysis in honeyChromatographia, 2001
- Analysis of Organophosphorus Pesticides in Honeybee by Liquid Chromatography−Atmospheric Pressure Chemical Ionization−Mass SpectrometryJournal of Agricultural and Food Chemistry, 2001
- Solid-phase microextraction applied to the analysis of pesticide residues in honey using gas chromatography with electron-capture detectionJournal of Chromatography A, 1998
- Gas chromatography with electron-capture and nitrogen–phosphorus detection in the analysis of pesticides in honey after elution from a Florisil columnJournal of Chromatography A, 1998
- Determination of pesticide residues in imported and locally produced honey in JordanJournal of Apicultural Research, 1997
- Acaricide Residue Determination in HoneyBulletin of Environmental Contamination and Toxicology, 1996
- Simplified Procedure for Organochlorine Pesticides Residue Analysis in HoneyInternational Journal of Environmental Analytical Chemistry, 1994