An improved method for the simple and accurate colorimetric determination of urea with Ehrlich's reagent
Authors:
- H. YatzidisHYH. Yatzidis
- M. GaridiMGM. Garidi
- C. VassilikosCVC. Vassilikos
- D. MayopoulouDMD. Mayopoulou
- A. AkilasAAA. Akilas
Open Access
- 1 March 1964
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 17 (2) , 163-164
Abstract
An improved method for the colorimetric determination of urea is described. The method is simple, requiring a deproteinized filtrate obtained after treatment of a small quantity of whole oxalated blood, serum, plasma or urine with 12·5% trichloracetic acid containing 50 g. activated carbon. The addition of Ehrlich's reagent to this filtrate results in the immediate production of a stable yellow colour. The determination is accurate, very sensitive and rapid, taking place in only 10 minutes.Keywords
This publication has 3 references indexed in Scilit:
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- THE MANOMETRIC DETERMINATION OF UREA IN BLOOD AND URINE BY THE HYPOBROMITE REACTIONJournal of Biological Chemistry, 1929
- Über eine neue Harnstoffreaktion und Ihre Klinische VerwertbarkeitJournal of Molecular Medicine, 1922