Viability and respiratory activity ofPseudomonas syringaecells starved in buffer
Author:
- 1 April 1995
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 41 (4-5) , 372-377
Abstract
Pseudomonas syringae cells starved in buffer released orcinol-reactive molecules and materials that absorbed ultraviolet light. The number of cells culturable in nutrient medium decreased more rapidly than the number of intact particles determined by microscopy. The results suggested that starvation resulted in the lysis of an increasing number of cells, and that a fraction of the intact particles were not culturable. Starvation also resulted in a decrease in the rate of oxygen consumption with acetate, glycerol, and succinate, but at different levels. Whereas the respiration of acetate and glycerol decreased concomitantly with culturability, the respiration of succinate decreased to levels similar to the concentration of intact cells, suggesting that all intact particles respired the succinate, but only the culturable cells respired the acetate and glycerol. The results suggest that measuring the activity of the electron-transport system can overestimate the viability of starved bacterial cells, and that complex metabolic activities such as the respiration of acetate and glycerol are probably better suited for the evaluation of this parameter.Key words: Pseudomonas syringae, starvation, culturability, viability, respiration.Keywords
This publication has 26 references indexed in Scilit:
- Viable but non‐culturable salmonellas in soilJournal of Applied Bacteriology, 1993
- Factors affecting the selection and use of tetrazolium salts as cytochemical indicators of microbial viability and activityJournal of Applied Bacteriology, 1993
- Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environmentMicrobial Ecology, 1982
- The Survival of Coryneform Bacteria during Periods of Prolonged Nutrient StarvationJournal of General Microbiology, 1978
- Effect of Starvation on the Viability and Cellular Constituents of Zymomonas anaerobia and Zymomonas mobilisJournal of General Microbiology, 1970
- Degradation of Cell Constituents by Starved Streptococcus lactis in Relation to SurvivalJournal of General Microbiology, 1969
- Survival of Streptococcus lactis in Starvation ConditionsJournal of General Microbiology, 1968
- A Determinative Scheme for the Fluorescent Plant Pathogenic PseudomonadsJournal of Applied Bacteriology, 1966
- Oxidative phosphorylation in fractionated bacterial systems: VIII. Role of particulate and soluble fractions from Escherichia coliBiochimica et Biophysica Acta, 1963
- The Survival of Stationary Phase Aerobacter aerogenes Stored in Aqueous SuspensionJournal of General Microbiology, 1961