Immunoglobulin G- and M-specific enzyme-linked immunosorbent assay for detection of dengue antibodies

Authors:
  • DDD Dittmar
    D Dittmar
  • TCT J Cleary
    T J Cleary
  • ACA Castro
    A Castro

Abstract
An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conjugate and fractionated serum are employed. By using this technique, dengue immunoglobulin M antibodies were demonstrated in an infant suspected of having a recent dengue infection.

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