Fusobacterium nucleatum Subspecies Identification by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
- 1 April 2015
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 53 (4) , 1399-1402
Abstract
We explored the use of matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies ( animalis , fusiforme , nucleatum , polymorphum , and vincentii ) were analyzed and divided into four distinct clusters, including subsp. animalis , nucleatum , polymorphum , and fusiforme / vincentii . MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.Keywords
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