Quantitation of Adenovirus Soluble Antigens by Crossed Immunoelectrophoresis: Application to Serological Characterization of Mutants

Authors:
  • GMGuy R. Martin
    Guy R. Martin
  • RWRita Warocquier
    Rita Warocquier
  • PBPierre A. Boulanger
    Pierre A. Boulanger
Publisher website
Google Scholar
Abstract
The two-dimensional immunoelectrophoresis technique was applied to adenovirus type 2 soluble antigens. Electrophoresis of adenovirus-infected HeLa cell extracts in adenovirus antibody-containing agarose gel permitted us to assay for hexon, assembled penton, free penton base and free fiber simultaneously in one single experiment. This technique was routinely used to characterize the adenovirus type 2 temperature-sensitive mutants.