The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue
Top Cited Papers
- 16 September 2019
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 16 (11) , 1105-1108
Abstract
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample (www.mesospim.org).Keywords
This publication has 20 references indexed in Scilit:
- Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull–meninges connectionsNature Neuroscience, 2018
- A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearingNature Neuroscience, 2018
- Shrinkage-mediated imaging of entire organs and organisms using uDISCONature Methods, 2016
- Clarifying Tissue ClearingCell, 2015
- iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume ImagingCell, 2014
- Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational AnalysisCell, 2014
- Structural and molecular interrogation of intact biological systemsNature, 2013
- Three-dimensional imaging of solvent-cleared organs using 3DISCONature Protocols, 2012
- Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brainNature Methods, 2007
- Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination MicroscopyScience, 2004