Gut probiotic bacteria of Barbonymus gonionotus improve growth, hematological parameters and reproductive performances of the host

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Abstract
This study aimed to isolate and identify probiotic bacteria from the gut ofBarbonymus gonionotusand evaluate their effects on growth, hematological parameters, and breeding performances of the host. Five probiotic bacteria viz. Enterococcus xiangfangensis(GFB-1),Pseudomonas stutzeri(GFB-2),Bacillus subtilis(GFB-3),Citrobacter freundii(GFB-4), andP. aeruginosa(GFB-5) were isolated and identified using16S rRNAgene sequencing. Application of a consortium of probiotic strains (1–3 × 1.35 × 109 CFU kg−1) or individual strain such as GFB-1 (1.62 × 109 CFU kg−1), GFB-2 (1.43 × 109 CFU kg−1), GFB-3 (1.06 × 109 CFU kg−1), GFB-4 (1.5 × 109 CFU kg−1) or GFB-5 (1.43 × 109 CFU kg−1feed) through feed significantly improved growth, histological and hematological parameters and reproductive performances ofB. gonionotuscompared to untreated control. Moreover, the application of these probiotics significantly increased gut lactic acid bacteria and activities of digestive enzymes but did not show any antibiotic resistance nor any cytotoxicity in vitro. The highest beneficial effects on treated fishes were recorded by the application of GFB-1, GFB-2, GFB-3, and a consortium of these bacteria (T2). This is the first report of the improvement of growth and health ofB. gonionotusfishes by its gut bacteria.
Funding Information
  • Ministry of Science and Technology, Government of the People’s Republic of Bangladesh (2016-2017 (74 BS), 2018-2019 (224 BS))

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