Monomer Addition as a Mechanism of Forming Peptide Cross-Links in the Cell-Wall Peptidoglycan of Streptococcus faecalis ATCC 9790

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Abstract
The relative amounts of radioactively labeled disaccharide-peptide monomers and peptide-cross-linked dimers and trimers found in the peptidoglycan of S. faecalis ATCC 9790 were compared to the relative amounts to be expected from 2 different polymerization mechanisms (random condensation and monomer addition). Data from continuously-labeled, exponentially-growing cells are consistent with a monomer addition cross-linking process, not with a random condensation cross-linking mechanism. This was supported by analyses of cells labeled during valine starvation (and wall thickening), recovery from valine starvation and pulse and pulse-chase labeling of walls from exponentially-growing cultures.

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